User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/09

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Objective

We are running the same procedure as yesterday only with an inhibitor, EHNA.

Description

  1. Add experimental record here. Include what, how, and why...

Data

EHNA stock

  1. 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA
  2. (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA

The reaction samples will contain roughly 1nM EHNA

Notes

Creating the EHNA stock - 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)...(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA - The reaction samples will contain roughly 1nM EHNA UV/vis

Graph showing the changes in the concentrations of adenosine and inosine over time

Adenosine Concentrations vs. Time.png

Adding the EHNA appeared to slow down the conversion of adenosine to inosine.