User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/09/24
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To observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs.
The experimental details (pepsin cleavage of hemoglobin) are similar to this reference.
We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
Thank you to Carlina for making more Hemoglobin 5mg/ml for us.
Today we were able to complete the UV Vis for Pepsin at 0.5 hrs. We put all of our SDS page samples in the fridge, and tomorrow we will run a gel with those samples.