User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19

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Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

File:19102010 10% gel hTERT.tif

Attachment

<html> <a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Pierce resultaten HBE (& Jurkat)