User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/08

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Summary

  • run 1x 6% SDS-PAGE (prepared 1.10.2010) (2nd gel had bad comb)
6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(10 μL)
Jurkat
(30 μL)
HEK293
(30 μL)
HeLa -S
(30 μL)
Fermantas high range Marker
(10 μL)
S0
(30 μL)
S1
(30 μL)
X S2
(30 μL)
S3
(30 μL)
C0
(30 μL)
C1
(30 μL)
C2
(30 μL)
C3
(30 μL)
  • Run at 200 V
  • Prepared
    • Fixing solution (Coomassie)
      • 40% EtOH
      • 10% Acetic acid
    • Destain solution (Coomassie)
    • 10% Acetic Acid
      • NOTE: Could only find Glacial acetic acid (anhydrous acetic acid)