User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/05

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Summary

  • Cells for ELISA were stimulated with CSE and treated with db-cAMP or mPKI (all had at least DMSO) (15.30)
  • Cells for IF were stimulated with CSE with and without DMSO or Ht31 (15.55)
    • NOTE: Ht31 was diluted in S0 OR 15% CSE!! So no incubation with Ht31 prior to adding CSE!
  • New cells were split to P24 (4x 100mm dishes)