User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/30

From OpenWetWare
Jump to: navigation, search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngTrypsin/EDTA dilution, splitting cells for ELISA Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


  • EDTA solution
    • 2.5 mg EDTA in 10 mL PBS (0.05% EDTA)
    • 5.5 mg EDTA in 22 mL PBS
  • Dilute trypsin 5x
  • Cells were split to P28, 2x 24 wells for ELISA and 4x 10cm dishes for continuation
  • Immunofluorescence was looked at under microscope, it seemed quite fine
    • Cy3 signal of RII units was a bit faint, look into antibody or use different label
    • Use control were only secondary antibody is used!!