User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12

From OpenWetWare
Jump to: navigation, search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngAKAP250 (scbt) immunoblot AKAP250 Co-IP Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Summary

  • Washing the AKAP250 CO-IP Blot and adding secondary antibody
  • Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock)

Results

  • Immunoblot for AKAP250 on AKAP250 co-ip western blot

12072010 250co-ip AKAP250 Blot.png

Discussion

  • Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
  • Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on the ponceau of this membrane