User:Wesley J. Houston/Notebook/MAP/2013/03/18

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3/18/2013 Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 Concentrations: KAH201 = 48.1 ng/uL, KAH1182 = 6.3 ng/uL Concentrations were determined on 2/18/13 > Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng.   1 2 Insert DNA (KAH182) 8.0 --- Vector DNA (KAH201) 1.0 1.0 2x lgn buf (Roche) 10.0 5.0 T4 ligase (NEB) 1.0 1.0 dH2O 0 3.0   20 μL 10 μL Follow quick transformation protocol Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar Ligation Plate results (lig : neg crtl) 03/18/13 1. KAH201 (S/P)/3403, 50 ng + KAH182 (X/P)/1675, 50 ng 0 2. KAH201 (S/P)/3403, 50 ng 0