User:Wayne G. Shreffler/Notebook/Daily Log/2009/05/28

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*Wayne G. Shreffler 19:56, 28 May 2009 (EDT):

  • Went through R lesson with Shashi
  • Accepted ms from JACI to review
  • ~4 hours of patient work
  • reviewed Bert's fractions
    • Silverstain 052809B.jpg
    • Laverne will isolate more fraction 5 with 3 and 8 as controls
  • analyzed Bert's co-culture experiment:
    • 7-day co-culture of CFSE labeled autologous T cells with control, cpe, lps, or ct stimulated DCs together with increasing concentrations of retinol, retinal, or retinoic acid
    • no CCR9 induction
    • possible weak (very weak, ie wouldn't convince anyone) FoxP3 induction with exo RA in both CT and CPE
    • on the bright side -- clear induction of proliferation by CPE-stim DC over Medium