User:Wayne G. Shreffler/Notebook/Daily Log/2009/05/21

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*Wayne G. Shreffler 10:38, 21 May 2009 (EDT):Entry title

  • Finished and sent CoFAR Treg practicum analysis
    • need to update CFSE protocol to emphasize mixing for uniformity of staining
  • Finished reviewing Calman's paper 09-0565
    • would like to incorporate doublet exclusion and adopt his protocol for milk T cell responses in place of 48 hour cultures

  • Caitlin obtained promising DHR data showing induction of H2O2 by anti-IgE stimulation that could be completely blocked by SOD inhibitor and partially blocked by N-acetylcysteine
  • Media:DHR_052009.pdf
  • Bert obtained promising data showing induction of RA synthesis in peanut treated DCs using the Aldefluor assay