User:Ulyana Simakova/Notebook/Alien species Anadara kagoshimensis/2014/09/01
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Analyse existing data to create REALY specific primers
Using my data and data from Tanaka, Aranishi(2013, Genbank id's: AB854359.1-AB854417.1) and de Carlos, Banon(unpub, Genbank id's: KF417435.1-KF417440.1) Tanaka used his own primer as forward and HCO2198 as reverse. He accuired contig of 555 bp length of which first 8 bps are from primer (HCO2198)
4 differenences between. I Have to order new primer to try. Temperature differences between f&r are big but i dont see any difference with HCO2198 Also new primer can create a self dimers and hairpins but not really big.
And try to Use it