User:Torsten Waldminghaus/Notebook/Multiple Mutation Reaction/2008/04/29

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PvuII digest

  • Since the PCR was somehow not optimal (see yesterday) I just want to see if the PCR fragment can be cut with PvuII which should only cut when a right point mutation was inserted (see project dexcription).
  • digest:
    • 5 μL PCR product
    • 1.5 μL NEBbuffer 1
    • 1 μL PvuII
    • 7.5 μL A. dest

=> 1h 37 °C => add 5 μL sample buffer and freece at -20 °C