User:Tk/Notebook/MF/2009/11/22

From OpenWetWare
< User:Tk‎ | Notebook‎ | MF‎ | 2009‎ | 11
Owwnotebook icon.png Mesoplasma florum simplification <html><img src="/images/9/94/Report.png" border="0" /></html> Main Page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Cutting DNA with Sau3AI and SpeI

Tk-11-22-09.jpg

Cut 1 μg DNA in 50 μl in buffer 2, BSA, 1 μl enzyme


  • L1
    • Uncut
    • Sau3AI digest
    • SpeI digest
  • GF
    • Uncut
    • Sau3AI digest
    • SpeI digest
  • PP
    • Uncut
    • Sau3AI digest
  • Ent
    • Uncut
  • PP
    • SpeI
  • Ent
    • SpeI digest
    • Sau3AI digest


L1 cannot be cut with Sau3AI. Poorly cut with SpeI. Other strains are completely cut with Sau3AI, cut similarly with SpeI.