User:Tk/Notebook/MF/2009/11/21

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Cutting DNA with EcoRI and PstI

Tk-11-21-09.jpg

Cut 1 μg DNA in 50 μl in buffer 2, BSA, 1 μl enzyme


  • L1
    • EcoRI digest
    • PstI digest
    • E & P digest
  • GF
    • EcoRI digest
    • PstI digest
    • E & P digest
  • PP
    • EcoRI digest
    • PstI digest
    • E & P digest
  • Ent
    • EcoRI digest
    • PstI digest
    • E & P digest


L1 cannot be cut with PstI (note the methylation of CAG and CTG sites, preventing cutting) Other strains appear to be only partially methylated at these sites.