User:Tk/Notebook/MF/2009/10/01

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PCR of pWV01 origin

  • construction of registry J70500 part
  • PCR from
    • pMG36 colony 1 (Kan resistant, no plasmid?)
    • pMG36E colony 1 (Ery resistant)
    • pMG36C colony 1 (Cm resistant)
    • pMG36 colony 2 (Kan resistant, no plasmid)
    • pMG36E colony 2 (Ery resistant)
Tk-9-30-09.jpg
  • Prep of PCR from Ery resistant bands gives 67 and 89 ng/ul in 50 ul volume