User:Tk/Notebook/MF/2008/07/13

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More transformations

  • 10 ml cultures harvested and wash 2x with EPB, resuspended to 1200 ul in EPB
  • mixed with 16 ul of old transposome, 143 ng/ul; also added 8 ul of each new SB transposomes
  • mixed 80 ul 1 M sorbitol
  • 4 1mm cuvettes, 1.5 KV, one arc'd and was discarded
  • Added 1 ml 1161 (no tet)
  • incubate 55 min, slight color change
  • plate on 3 x 3 plates -- washed cuvettes with 200 ul 1161 and plated that also

Dishwasher is busted

  • again! no wash motor action, no heat