Construction plasmid PCR
- 100 ul final
- 50 ul Phusion mastermix
- 1.5 ul suffix-FB
- 1.5 ul prefix-RB
- 0.5 ul of plasmid (pSB1AC3, 1AK3, 1AT3)
- 46.5 ul water
- Cycle 98/30s 32x(98/20s 55/20s 72/2min) 72/10min
Transformation results
- Picked 40 colonies off of 4/20 transformations
- +10 worked best, 0 worked well, -5 few, -10 none, +15 few
- replated 0,-5 saved cultures
Construction plasmid distribution
- Each team gets 300 ul of each plasmid
- Concentration is 10 ng/ul
- Each team needs 3 ug of plasmid
- With 150 teams, we need 450 ug of each plasmid
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MF-xfm
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