User:Tk/Notebook/MF-xfm/2008/04/14

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Transformations

  • Some small colonies of a few of the older plates
    • likely junk

PCR of transposons

  • 20 ul reactions
  • 0.6 ul ME0 primer
  • cycle 95/5 min, (95/20s, 55/20s, 72/3.0 min) x 32
  • wells:
    • 83 ng/ul cut PCR'd transposon
    • transposome
    • cells from culture B of initial transformations (streaked plate for growth test)

Setup of cultures for tomorrow

  • 50 ml cultures infected with
    • 5 ml
    • 0.5 ml
    • 50 ul
    • 5 ul