User:Tim Henry/Notebook/Family portrait/2011/07/09
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Multiplex PCR attempt 1
First attempt putting multiple primers into a single reaction. Apparently this will save money and give me the results I'm looking for. The negative being that some of the primers chosen have products that are very close in size and create doublet/triplets.
Start 1:30pm - Done 3:36pm
59 (Initial extended) didn't auto switch program to 60, so 6min at 94°C accidentally.
60mL TAE + 1.2g agarose (2%)
Electrophoresis 3:49pm, 50v
End 6:40pm. Started stain, 7:08 destain