User:Tim Henry/Notebook/D1S80/2012/02/20

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D1S80 w/ LB buffer

D1S80 gel run on 2012/02/20

Chelex Buccal extraction 2012/02/18

PCR on 2012/02/19


First time using LB buffer.

1.1% agarose. 75mL total. (.825g agarose, + 75mL LB)

280v for approx 45minutes. LB doc suggested 4x normal voltage.

Results look very smudged and much less defined than TAE version (suprising, was expecting cleaner bands).



Thenry D1S80 20120220.jpg