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I have made a sub.vi that can calculate the length of the microtubule from the opening moments of the tracking and at the very end. it can do this through the velocity and from measuring the distances between points. So it gave 4 different measurements--which are actually independent i believe.
But I wanted more than 4 so, i came up with this
This needs to smooth the complete trajectory between the front and end trajectories. It then calculates the distance between front and end for each frame.
I just want to run this by you guys and see if it is something you wanna put in. I should also put in the time array so i can calculate length through velocity as well.