# User:TheLarry/Notebook/Larrys Notebook/2009/11/13

Generating Data | Main project page Previous entry Next entry |

## Gaussian Point Spread FunctionSo my point spread function will be approximated as a Gaussian. It can be an Airy Disc but i still don't know how to make a 3-d airy disc, so for now I'll use a Gaussian. After today's group meeting Koch helped me out with some troublesome parts of this simulation - To find the spread for the Gaussian, I can just look at a picture of the quantum dot and match the spread for that
- To find the amplitude best just fit as best as i can by eye
- For the placement of the fluorescent dots I can split up the microtubule into x number of spots (Koch can you write down how to split this up again, i forgot), then roll the dice for each spit giving it a 17% chance that the dot will be at that location. 17% is the percentage of concentration in the solution.
Right now i have the Gaussian spread to be about .09 which looks pretty good but i am unhappy with this procedure there must be a more precise way to do this. I just don't know how, and every where i look this part seems glazed over ## Airy DiscoOn the bright side i got my 3-d Airy Disc going. Hooray for me. Now i can use this instead of the Gaussian for my point spread function. That is good news. This opens up the question now of how do i deal with the smear. before i didn't know what i was doing but was confident i know what sigma of the Gaussian is. Now I have this Airy Disc and I am not 100% confident i know what to change to match experimental conditions. Here i'll write down what i do know: Oh so i don't forget Andy said there are probably some software packages that figures out the PSF already. That might be worth looking into if this starts to drive me crazy. But i feel right now that i am on the right path. I'll fight it but i probably have to read a book or a chapter or something to get this right. OK I'll read Born/Wolf chapter on Airy Discs. Chapter 8. Hopefully that gives me some background to play with this function. I am keeping my fingers crossed that the wavelength and aperture size is all i really need to match the images from the microscope. It could be -- i wanna believe (also [math]\displaystyle{ I_0 }[/math]) Also if any one knows this aperture size please feel free to put it here |