User:Tara K. Luckau/Notebook/Team ConGen/2013/04/17

Tara's Lab Notebook

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SCOC MP2 State of the Multiplex Summary of Past Results SW614- A4, A6, B1, B12 gelled well amplified well in uniplex amplified well in multiplex with all 8 SW markers multiplex with A4 + A6 + B1 + B12 gel blank frag fail  ?! maybe due to Techne cycler? re-do of multiplex with A4 + A6 + B1 + B12 (didn't run gel) frag failed again! ran on Techne again - thermalcycler poopy? re-re-do of multiplex with A4 + A6 + B1 + B12 gel blank frag failed AGAIN! ran on Opticon  :( Next Steps redo it ALL to figure out what the problem is potential problems: DNA - run 4 samples, not just the 2 controls fluorophore - can re-order labelled primers, but only after testing other options because of price primer interactions - none to be concerned about cycler - avoid using Techne until properly tested 3730 - Crystal reports no problems with other clients' data uniplex/4-plex/8-plex - just re-do these reactions Taq - can run 2 replicates using 2 different lots of Taq, but don't know what lot I used for the above failures, so not a good test Buffer - don't know how to test tackle the bolded potential problems first (#s 1 and 6)