SCOC MP1-G
Gel
- decreasing gradient of Scun15 concentration (peak too high) (Scun9 and Scun19 concentrations held constant)
Pour |
Load |
Run
|
270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
140 V
|
27 µL GelRed |
6 µL ladder |
50 minutes
|
- three distinct bands visible for most conditions; bands occur in expected size ranges
- differences between the two control DNAs are evident, and not expected - hopefully results will look consistent in fragment analysis regardless of source DNA
- from gel view, predict 0.075µL/reaction Scun15 will be preferred
- OVERALL: gel looks good - send to frag
|