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SCOC Multiplex Optimization
Fragment Analysis Results
SCOC MPY Uniplexes
Scun3
Scun11
sar80
SCOC MPG
for evenness
- this is the best sample; most had extremely high Scun15 and extremely low Scun19, low Scun9
+ sar80
- sar80 did not amplify at all when multiplexed with the green lane
SCOC Conclusions
- Scun11 range is very large and overlaps with sar80
- sar80 seems non polymorphic?
- because of sar80's resistance to amplify and overlapping amplicon range with Scun11, needs to be run separately from the other 9 markers for SCOCs
- may be able to integrate it with the 8 SW markers not yet screened
CNHY Multiplex Optimization
Fragment Analysis Results
CNHY MPA
throw it all together to see what happens
- this is by far the best sample: many markers seemed to amplify (Ai5033, Ai5037, Acos5, Ai5035, Ai5062), only Ai5057's amplicon is not seen
- all other samples only amplified Acos5
CNHY Conclusions
- oye, lots of work still to do, here
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Tara's Lab Notebook
Main project page
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