User:Tara K. Luckau/Notebook/Team ConGen/2012/03/27

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SCOC Multiplex Optimization

PCR

SCOC MPY

  • Problem: marker ranges in yellow lane of SCOC Multiplex may be wider than currently set (particularly Scun11)
  • Experiment: run Scun3, Scun11 and sar80 in uniplex on wide range of samples to establish marker ranges
  • 20120327 PCRa.png


SCOC MPG

  • Problem: Scun19 showing up low intensity
  • Experiment: decrease tall Scun15, increase short Scun19 to even out peak heights
  • 20120327 PCRb.png


SCPC MPG + sar80

  • Problem: sar80 seems to want to be run in uniplex because it doesn't play well with others in the sandbox
  • Experiment: try to stick sar80 primers into MPG (same buffer and annealing temperature as MPY) to prevent having to run sar80 in uniplex always
  • 20120327 PCRc.png


CNHY Multiplex Optimization

PCR

  • start trying to group markers together into multiplexes
  • start with same volume of each primer (can modify primer volumes based on results of this PCR)


CNHY MPA

  • Ai5033, Ai5057, Ai5037, Acos5, Ai5035, Ai5062
  • 20120327 PCRd.png


CNHY MPB

  • Ai5043, Acos3, Ai5013, Ai5071
  • 20120327 PCRe.png