User:Tara K. Luckau/Notebook/Team ConGen/2012/01/19

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CNHY / CNTI Multiplex

PCR

  • 20120119 PCRa.png


SCOC Multiplex

PCR

  • 20120119 PCRb.png


Gel

  • used gel made 17Jan2012


Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 140 V
27 µL GelRed 6 µL ladder 40 minutes


20120119 Gel.png

  • in CNHY / CNTI Uniplexes, 2 out of 3 amplified; but previous uniplex amplified well, so we'll go with it
  • in SCOC Scun10 trials, good banding, water is clean


  • send to frag!


Fragment Analysis Submission

  • 20120119 Frag.png


20120119 FedEx.png
  • UAGC Submission# RRAX


  • FedEx picked up 5:26pm, 19 January
  • FedEx delivered 9:18am, 20 January
  • UAGC received 10:21pm, 20 January
  • UAGC completed 3:00pm, 20 January