|
CNHY Ai5037
PCR
Gel
| Pour |
Load |
Run
|
| 270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
160 V
|
| 27 µL GelRed |
6 µL ladder |
45 minutes
|
- favorable conditions indicated by
Meeting with Bohonak
Proposal stuff
- overall, what I have right now is sufficient for a proposal, so long as I can defend the remainder of the work
- some tables:
- one with loci (each species) ... # proposed, # potential, # screened, # polymorphic, # polymorphic/need cleaning, # monomorphic/dirty/throw out
- one with tissue (each species / location) ... # proposed, # captured, # extracted
- mendelian inheritance of markers:
- families
- if not, then gravid female
- if not, then H-W equilibrium (and the thing from Genelex), with defense of why this is good enough
- calculation of error rates:
- present a plan for calculation
- some citations from Justin's: Hoffman and Amos; Bonin et al 2004
- handful of samples re-extracted, re-amplified, re-scored throughout the process (not all at the end)
- because of human error, null alleles
- lay out a plan for hypothesis testing
- for hypothesis 1, here are the three analyses I'll be using
- for hypothesis 2, here are the three analyses I'll be using
- be able to talk about assumptions of each analysis program
- popgen knowledge should be sufficient
- familiarize with each individual program
CNHY Ai5042
PCR
|
Tara's Lab Notebook
Main project page
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