User:Tara K. Luckau/Notebook/Team ConGen/2011/10/24

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CNHY Acos6

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V ... 80 V
27 µL GelRed 6 µL ladder 25 min ... 1.5 hr


20111024 Gel.png

  • lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions

CNHY Ai5013

PCR

  • 20111024 PCR.png