User:Tara K. Luckau/Notebook/Team ConGen/2011/10/20

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Tara's Lab Notebook Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


CNHY Acos2

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20111020 Gela.png

  • mispriming everywhere - primer pair not preferable for CNHY
  • if needed, may be able to use conditions noted by "20111020Tri.png"
  • 20111020Tri.png = "ehh, not great, but not horrible, either"


CNHY Acos3

PCR

  • 20111020 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20111020 Gelb.png

  • favorable conditions noted with Greenhappyface.png


CNHY Acos5

PCR

  • 20111020 PCRb.png


CNHY Acos6

PCR

  • 20111020 PCRc.png