User:Tara K. Luckau/Notebook/Team ConGen/2011/09/30

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sar84

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20110930 Gela.png

  • mispriming everywhere - primer pair not usable for SCOC


SA52.33

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20110930 Gelb.png

  • mispriming everywhere - primer pair not usable for SCOC


SA60.02

PCR

  • 20110930 PCR.png