User:Tara K. Luckau/Notebook/Team ConGen/2011/09/23

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sar82

PCR

  • 20110923 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20110923 Gel.png

  • mispriming everywhere - primer pair not usable for SCOC

sar80

PCR

  • 20110923 PCRb.png