User:Tara K. Luckau/Notebook/Team ConGen/2011/07/01

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Tara's Lab Notebook Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


SCOC MP1 Frag Data

Diversity Panel Analysis

  • each sample was replicated 4 times ... shown is a representative from the replicates
  • Impressions:
    • ladder looks great!
    • some things worked
    • a bit of pull-up/bleed-through
    • variability between samples - yay!
  • Preliminary Scores:
    • 20110701 FragScores.jpg
    • HOL and LOM did not amplify
    • TP3 had an extraneous peak in the green lane - could possibly be Scun3

Water

  • 20110701 FragH2O.jpg

RAJ

  • 20110701 FragRAJ.jpg

HOL

  • 20110701 FragHOL.jpg

SYR

  • 20110701 FragSYR.jpg

CPN

  • 20110701 FragCPN.jpg

TP1

  • 20110701 FragTP1.jpg

TP3

  • 20110701 FragTP3.jpg

LOM

  • 20110701 FragLOM.jpg

Multiplex Analysis

Lane Overlays

  • shows how all samples clump together
  • red circles indicate marker locations
    • blue lane bleeds through to green lane
  • 20110701 FragBlue.jpg
  • 20110701 FragGreen.jpg


  • zoom in on a particularly pretty sample (SCOC SYR2 X01 b)
  • trying to identify the markers:
    • going by color, the three markers that worked in this multiplex should be Scun2, Scun10 and Scun11
    • going by size, as compared to the gel from 23 June 2011, the three markers that worked in this multiplex should be Scun3, Scun11 and Scun15 (if the dyes got shifted)
  • 20110701 FragSCOCMP1 3markers.jpg
  • here's the map from 14 June 2011:
  • 20110614 SCOCMP1Map.jpg