User:Tara K. Luckau/Notebook/Team ConGen/2011/06/07

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Scun10

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 160 V
27 µL GelRed 6 µL ladder 1 hour


  • double banding noticeable under majority of PCR conditions; I'm assuming this is attributable to diploid nuclear DNA - in this case, a large enough difference in repeats between the two copies that it's clearly visible on an agarose gel
  • 20110607 Gel.jpg


Scun11

PCR

  • 20110607 PCR.jpg

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 140 V
27 µL GelRed 6 µL ladder 1.5 hour


  • 20110607 Gel2.jpg

Scun14

PCR

  • 20110607 PCR 2.jpg