User:Tara K. Luckau/Notebook/Team ConGen/2011/03/04

Tara's Lab Notebook

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Scun22 Optimization PCR Gel from 23 february 2011 PCR (full temperature and buffer gradient) Gel Specs Pour: 270mL 1xTAE + 5g agarose (supposed to have been 5.4g) + 27µL GelRed Run: 40 min @ 110V Gel Images Only Buffer G yielded any product ... zoom in: Conclusions doesn't look like a typical MgCl2 and temperature gradient PCR inconsistent banding patterns across temperatures with dropouts regardless, there is mispriming in all conditions that yielded any product Scun22 might not be a primer that will work for Sceloporus occidentalis