User:Tara K. Luckau/Notebook/Team ConGen/2011/01/10

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PCR Touchdown

  • to duplicate PCR conditions of primers' publication (Lance et al 2009)
  • PCROpt Touchdown.jpg

PCR Timber Rattlesnakes

  • to have a positive control
  • Rulon did a PCR on his Timber rattlesnake samples, using all his reagents (except for Taq)
    1. Rulon's dNTPs
    2. Rulon's 10x PCR Buffer
    3. Rulon's MgCl2
    4. Rulon's primer 2 and primer 10
  • PCROpt TimberRulon.JPG