User:Tara K. Luckau/Notebook/Team ConGen/2010/11/05

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Gel Scun2 F-R with DNA Gradient

Cast Gel

  • 270µL 1x TAE + 5.4g agarose
  • hot plate + stir bar
  • 27µL Gel Red (added when agarose turned clear but still hot, used stir bar)

Load Gel

  • 5µL loading dye + 5µL sample
  • 20101105 GelSchematic.jpg

Run Gel

  • 130V, 2hrs

Image Gel

Results

  • Very faint bands at E2, E4, E6, corresponding to DNA concentration of 1x, Buffer G, Temperatures 55, 57, 59
  • around 150 bp band