User:Tara K. Luckau/Notebook/Team ConGen/2010/10/21

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Gel Scun2 F-R

Make 1x TAE

  • 20mL 50x TAE + 980 mL NanoPure H2O

Cast Gel

  • 100mL 1x TAE + 1.5g agarose
  • large stirbar, hotplate set at 150 for about 30 min
  • let cool about 20 min
  • not enough volume! FAIL!

Next Try

  • 270mL TAE + 4.05g agarose
  • (for future reference, total volume ≈ volume of gel box in cm3, ie 27cm long x 20 cm wide x 0.5 cm thick gel = 270mL of TAE)