User:Tara K. Luckau/Notebook/Team ConGen/2010/09/27

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Ordered Primers

  • Ordered first set of primers
  • Scun2 and Scun22 (highly variable simple motifs)
    • Lance et al (2009) Conservation Genetics Resources, Sceloporus undulatus
  • Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT)
  • Luckau Primer20100927.jpg
  • Try all four combinations
  1. F - R
  2. F - R pigtail
  3. F M13 - R
  4. F M13 - R pigtail
  • If they don't hinder efforts or change optimization decisions, then may make optimization of multiplexes cheaper!