User:Tamra L. Fisher/Notebook/Research for Dr. Hartings/2012/03/28

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Beginning Expression

  • Add one colony from the transformation plate (BL21(DE3)+M33S/M103S - 3/26/12) to 35mL of LB with 100μg/mL ampicillin (four of these)
  • Incubate these overnight at 37°C at 250rpm.