User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/18

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Objective

Analyze the intein reactions that were set-up yesterday.

Description

  1. Take fluorescence spectra of the MBP-intein reactions with the gold nanoparticles from yesterday (tubes 7-12).
  2. Also take fluorescence spectra of unreacted (mixed in cuvette, and then immediately placed in the fluorometer) MBP-intein and gold nanoparticle mixtures.

Data

9x dilute.png

20x dilute zoomed.png


30x dilute.png

For the graphs where the "not reacted" data line is not easily seen, it follows the same line as "reacted at 37°C"

Notes

There is a major problem with getting protein gels (SDS-page) to polymerize for use. It would have been helpful to have seen the reaction mixtures on a gel.