User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/14

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Objective

Begin expression of mutated GFP (D1C) in BL21(DE3) E.coli.

Description

  1. 25μL of 100mg/mL ampicillin was added to 25mL of LB (four of these).
  2. One colony from yesterday's transformation was added to each of these flasks.
  3. The flasks were incubated overnight at 37°C at 250rpm.