User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/11/16

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png AU Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


To see if the peak will shift for the purplish-pink solution (previously synthesized gold nanoparticles) at a neutral pH and at different temperatures.


  1. Mix 750μL purplish-pink solution (made previously) with 750μL 1M Tris Buffer, pH 7.5 in a microcentrifuge tube. Make two of the mixtures.
  2. One of these microcentrifuge tubes will be stored at 4°C and the other at 80°C.
  3. Take UV-Vis spectra of each of these solutions every half hour from 400-750nm.


  • The spectra of the solution in heat and cold over time:


(Ignore the lines at x=0 along the bottom of the graph)