User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/11/16

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To see if the peak will shift for the purplish-pink solution (previously synthesized gold nanoparticles) at a neutral pH and at different temperatures.


  1. Mix 750μL purplish-pink solution (made previously) with 750μL 1M Tris Buffer, pH 7.5 in a microcentrifuge tube. Make two of the mixtures.
  2. One of these microcentrifuge tubes will be stored at 4°C and the other at 80°C.
  3. Take UV-Vis spectra of each of these solutions every half hour from 400-750nm.


  • The spectra of the solution in heat and cold over time:


(Ignore the lines at x=0 along the bottom of the graph)