User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/11/09

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Objective

Synthesize gold nanoparticles without purple protein fibers.

Description

  • Place three test tubes in a water bath at 80°C
    • test tube 1: 50μL BSA + 50μL HAuCl4 + 900μL H2O
    • test tube 2: 50μL HAuCl4 + 100μL BSA + 850μL H2O
    • test tube 3: 80μL BSA + 80μL HAuCl4 + 640μL H2O
    • After two hours let water bath cool overnight
  • Place three test tubes in heat block at 80°C
    • test tube 1: 50μL BSA + 50μL HAuCl4 + 900μL H2O
    • test tube 2: 50μL HAuCl4 + 100μL BSA + 850μL H2O
    • test tube 3: 80μL BSA + 80μL HAuCl4 + 640μL H2O
  • Place test tube (80μL BSA + 80μL HAuCl4 + 640μL H2O) in thermocycler
    • run at 80°C for two hours then decrease 2°C every 2 min until the sample reaches room temperature
  • Run microwave reaction with 400μL BSA + 400μL HAuCl4 and water to the top (about 8 mL) at 300 W at 80°C for 30 min
    • Allow full cool down time

Data

  • Images of tubes from today's experiment (left to right: tube in thermocycler, tube #3 from heating block, tube #1 from heating block, tube #2 from heating block):

053.JPG 054.JPG 055.JPG 056.JPG

This data showed that increasing the ratio of BSA/gold will prevent the formation of purple protein fibers.

Notes