User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/10/04

AU Biomaterials Design Lab

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Objective To find the sensitivity of the fluorometer using Ru(bpy)(phen) in acetonitrile. Description and Data First, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM: Standard Amount of Ru(bpy)(phen) Amount of acetonitrile Final Concentration in Cuvette Standard 1 60 μL 2940 μL 7.2 μM Standard 2 120 μL 2880 μL 14.4μM Standard 3 180μL 2820μL 21.6μM Standard 4 240μL 2760μL 28.8μM Standard 5 300μL 2700μL 36μM Standard 6 600μL 2400μL 72μM Standard 7 900μL 2100μL 108μM The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed: The curves were integrated in Excel and the following areas under the curves were found: Concentration (μM) Integrated Intensity 7.2 682.1927 14.4 886.9565 21.6 1091.243 28.8 1339.258 36 1436.954 72 1767.737 108 1877.2334 These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument: The calculated integrated intensities for all data collected from the class was graphed in the same way: Notes