User:Tamanika Tinsley/Notebook/CHEM 605 SHMP Studies/2015/10/14

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Oct 14 - HPLC Introduction

Sample Prep

  • ~120ppm stock solutions of SHMP from three locations were made
  1. Yanbu 0.0118g to 100g DI water
  2. Ash 0.0119g to 100g DI water
  3. AP 0.0121g to 100g DI water

Instrument

  • Waters 1525 Binary HPLC
  • Waters 2489 UV/Visible Detector
  • Waters 2414 Refractive Index Detector

Notes

  • Start with instrument >setup > loads> run > equilibrate > auto-zero < 250 µl <must filter samples
  • Internal Temperature should be higher
  • Headecyltrimethyl ammonium bromide C19H42BrN MW 364.45 g/mol
  • 50/50 water Acetonitrile CTAB
  • Made ~800ml of 10milimole CTAB

Results

  • Water

Water 10142015 waters uv.jpg

  • CTAB

CTAB 10142015waters UV.jpg

  • ASH SHMP

ASH SHMP 10142015 waters UV.jpg

  • Na3PO4

10142015 120ppm Na3PO4.jpg