User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/12/07

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Objective Determine the effect of adding a refolding buffer to fiber forming reaction before removing from heatblock. Examine gradient reactions from 12/6/11 in UV-Vis Description An AuNP synthesis reaction was run using the same concentrations as reaction 1 from 12/06: 100μL 17.7μM BSA, 100μL 2.9mM Au, 800μL water in a plastic tube. The reaction tube was place at 80°C in a heat block. On 12/06 this reaction formed fibers when removed from the heat block. Today, 500μL 50mM Tris, pH 7.5., used as a refolding buffer, was added after three hours. Five minutes after the refolding buffer was added the reaction was removed. No fibers formed. Data Peak locations: Au/BSA 164- 544nm Au/BSA 131- 533nm Au/BSA 66- 535nm Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.