User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/10/26
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De-stain SDS-PAGE protein gel.
Repeat AuNP snythsis reaction in water and run a control reaction of just BSA in water
Reaction 1- 1ml 15μM BSA + 2ml 0.75mM HAuCl4 removing from heat for 10 minutes every 30 minutes
Reaction 2- 1mL 15μM BSA removing from heat for 10 minutes every 30 minutes
Remove Coomassie Brilliant Blue R250 from gel
Add de-staining place on shaker
When de-staining solution has become saturated, remove and replace
Leave of shaker overnight
BSA and Water Only- No Observed Change
AuNP Synthesis- 1st observed gold colored fluid aggregating near the top of the solution, then black fibers apeeared and balled together.
This area is for any observations or conclusions that you would like to note.