User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/10/18
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Make a labeled cysteine fluorophore.==Description==
5mg 5-iodoacetamido fluorescein, 19.6μL 118mg/mL cysteine, and 35mM Tris, pH 7.5 were placed in a 50mL Falcon tube.
Cystein to fluroscein 5mg fluroscein (?) 1.17mg cyesteine 1:1
in 35mL buffer 50mM Tris ph 7.5
Absorbance of 20X dilute at 490= 1.144 Molar absobtivity= 42,000 1st paper
2.77*10^-4 mol/L if we had used 5mg
1.907mol fluroscein = 9.8mg
2.31mg cysteine add 1.14 more
stock solution 118mg/mL = 9.6microliters
added another 9.6microliters for a total 19.6microliters of stock cysteine
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