User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/09/06

Protein Film

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Objective Formation of a zein protein film. Description A 90% v/v aqueous ethanol solution was prepared using 9mL of ethanol and 1mL of water. 0.237mL of glycerol was added to 1 g of zein. The 90% ethanol solution was then added to the mixture of glycerol and zein to make a final 10mL solution. The solution was gently mixed and then 5g was transfered to a 9cm diameter Petri dish and place in a 40°C oven for 24 h. Data 10% (w/v) zein in 10mL solution - 1g zein 30% glycerol by zein weight- 0.3g glycerol * 1mL/1.261g = 0.237mL glycerol Notes When observed at 1:15pm on 9/7/11, a thin yellow film had formed. When veiwed under the microscope small particles were dicernable. The film was removed from the dish and it was smooth and flexible. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.